1. Do your products have quality assurance?

To ensure that the quality of each product is qualified, it is given with a COA and HPLC, NMR test result.

2. How should the products be handled once they've been received?

During shipping, the product package may be bumped, causing the reagents to stick to the tube wall or cap. Do not open the cap immediately after removing the tube from the box; instead, gently shake it to allow the reagent to fall to the bottom of the tube. Centrifuge liquid products at 200-500 RPM and collect the liquid at the tube's bottom. Attempt to avoid product loss or contamination during processing.

3. Orders are delivered outside of business hours and left at room temperature, with labels indicating that they should be stored at -20°C. Is the product going to go bad?

The storage temperature indicated by the label on the tube is optimal for long-term storage. Most compounds are very stable in the short term at room temperature.

4. Why do the tubes in my package appear to be empty?

When a tiny amount of product is purchased and some of it sticks to the tube wall during lyophilization to produce a thin coating, the existence of the product may go undetected. To dissolve it entirely, add the necessary solvent (according to the operating instructions) and vortex or ultrasonically shake it.

5. How do I remove waxy solids or hygroscopic compounds from my compounds for weighing and storage?

We propose dissolving waxy or greasy items directly with a suitable solvent if they are difficult to remove and weigh; hygroscopic chemicals that collect moisture when exposed to air and appear as droplets should be stored in a desiccator.

6. What is the best way to dissolve the compound?

By consulting our product instructions, you can determine which solvent to use.You can choose the solvent by referring to our product manual. Most products are not directly soluble in water, PBS buffer or saline, you can first prepare storage solution with DMSO or ethanol, which are usually able to be stored for a long time and can be diluted with aqueous solution to the concentration required for in vivo and in vitro experiments. In most cases, the dissolution of the product in these organic solvents is high enough to ensure that the concentration is adequate for the experiment after dilution at 1:500 to 1:1000 with aqueous solvents. Usually, small amounts of organic solvents (e.g., 0.1% DMSO) do not affect biological experiments, but we recommend that you establish a solvent control group at the time of your experiment.

7. Compounds dissolve well in DMSO, but solids precipitate when diluted with aqueous solvents?

This phenomenon is very common and the precipitated solids can be redissolved after a few minutes of vortexing, sonication and water bath heating to 37°C. Please make sure that the precipitation has completely dissolved when using the solution.

8. The instructions state that the compound can be dissolved at a specific concentration, but the compound  is still not completely dissolved, what should I do?

Please heat the solution to 37°C and vortex or sonicate it for a few minutes, usually the compound can be completely dissolved after this treatment. If this does not completely dissolve the compound, please contact our technical support for further assistance.

9. What should I do with any remaining product if I won't be able to utilize it all at once?

Most of the products can be formulated into DMSO or other organic solvent masterbatches, which can be stored at -20°C for 3 months. 5 repeated freeze-thaws or less will not significantly affect the biological activity of the product. At the same time, some products are unstable in solution and need to be prepared ready for use. For these products, you need to prepare the solution before use and use it up within 24 hours.

10. How do I prepare the stock solution?

Choose a suitable solvent to prepare the stock solution according to your experimental protocol. The solubility information measured by Taosu Biochemistry is displayed on the product page of the website. DMSO is the most commonly used solvent for stock solution preparation in in vitro experiments. The stock solution is then diluted into a working solution (e.g. 1:1000 in cell culture medium) before use.